تعبير البروتين refers to the synthesis of البروتينات within cells using information contained in the DNA or gene.
البروتينات are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study بروتين function in order to understand cellular processes.
This is currently studied based on using fluorescent البروتينات as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for البروتينات التي هي عابرة أو سريعة الزوال بطبيعتها.
أبلغ الباحثون عن تقنية جديدة على خادم ما قبل الطباعة في 30 يوليو 2020 والتي يمكنها التغلب على هذا القيد.
The new study describes the use a fluorescent biosensor that enables real-time detection of بروتين expression in vivo which has implications for understanding the spatiotemporal expression of البروتينات within the living organism. This sensor is based on a dim green fluorescent بروتين in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a بروتين tag and enables detection of بروتين expression within seconds in live cells.
هذه جهاز الاستشعار البيولوجي will be useful to study biological processes in real-time where البروتينات are either transiently expressed and/or to understand the expression and interactions of البروتينات from a disease-causing organism such as bacteria or a virus with that of the host proteins.
مرجع:
Eason MG. ، Pandelieva AT. ، Mayer MM. ، et al 2020. مستشعر حيوي فلوري مشفر وراثيًا للكشف السريع عن التعبير البروتيني. الطباعة المسبقة: bioRxiv 2020.07.30.229633 ؛ DOI: https://doi.org/10.1101/2020.07.30.229633
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